Yearly Archives: 2024

　　Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .In the eyes of industry experts, pu-erh tea Indeed, it has great development potential, which makes many investors more interested.

　　When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

　　The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

　　Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

　　There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

　　The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

　　Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

　　Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

　　Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

　　What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

　　Separation and purification of protein;from small molecules Looking at the development prospects, the future will always bring positive effects. https://www.all-chemistry.com/

　　1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

　　2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

　　3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

　　4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

　　5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

　　Amino acid chain is the main and core component in protein, but it is not necessarily the only component. Some protein may include some atoms or small molecules, whose function may be to perform protein function and/or increase its stability. Chemically, these cofactor are diverse, which may be organic molecules or metal elements. Some of them are briefly combined with protein chain, while others are closely combined with protein chain (sometimes even by covalent bonds) and become an integral part of the whole protein. The former is called coenzyme coenzyme, while the latter is called prosthetic group. Most coenzymes are small organic molecules from vitamins. For example, the common coenzymes NADH and FADH2. These two molecules from vitamin B complex, the former from nicotinic acid (vitamin B3) and the latter from riboflavin (vitamin B2), play the role of high-energy electron acceptor/donor in redox reaction.To some extent, recombinant proteins Our development has surpassed many peer businesses, but it has never stopped moving forward. https://www.alphalifetech.com/

　　Some enzymes use several different cofactors, such as Pyruvate dehydrogenase (PDH), which is a key enzyme in the catabolism of sugar. The catabolism of sugar begins with glycolysis, which is a biochemical reaction pathway with 10 steps, transforming one glucose molecule into two pyruvate molecules, and then carrying out the citric acid cycle of tricarboxylic acid, which further decomposes pyruvate and oxidizes it into CO2. Pyruvate dehydrogenase PDH works at the intermediate junction of the above two reaction pathways (namely glycolysis and triacid cycle), and is responsible for activating pyruvate and enabling it to enter the tricarboxylic acid cycle. Specifically, PDH catalyzes the oxidative dearboxylation of pyruvate and forms acetyl coenzyme A (ACoA), which is the activated form of pyruvate. PDH is not a single enzyme but a complex composed of three components, each of which participates in a different step of pyruvate activation and uses different coenzymes. The first component uses thiamine pyrophosphate (TPP), which is a derivative of thiamine (vitamin B1). TPP allows the first component of PDH to be oxidized and decarboxylated at the same time, thus transforming pyruvate into acetyl, and the remaining carbon is discharged as CO2. The importance of TPP will be reflected in its absence. In fact, if people can’t get enough thiamine from food, they will get beriberi, which will damage several major systems of the human body.

　　The second component in PDH uses lipoic acid. It is an auxiliary group covalently bound to PDH. The active part of lipoic acid is a cyclic structure, which contains two covalently bonded sulfur atoms (that is, S-S bonds) (Figure 2.2c). This active part catalyzes the transfer of acetyl to coenzyme coenzyme A(CoA), the third cofactor of PDH. CoA is pantothenic acid, also known as the derivative of calcium pantothenate. The combination of acetyl and pantothenic acid involves the reduction of S-S bond, and its cyclic structure will open and form two sulfhydryl groups (one SH), one of which will bind acetyl. Sulfhydryl group is easy to react chemically, which can be reflected in the fact that sulfhydryl group is easily influenced by arsenic, a toxic derivative of arsenic. To sum up, the formation of acetyl coenzyme A involves the reduction of S-S bond of pantothenic acid cyclic structure. In order to keep PDH active, these two sulfur groups must be re-oxidized to restore the S-S bond.

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.among pu-erh tea It has given great spiritual support to entrepreneurs, and more entrepreneurs will contribute to this industry in the future.

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　And so next time, may is so every time.

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　”Domestic mechanical or quartz? What brand do you want?”

　　”To quartz, need not everyday winding, brand to the seagull.”

　　”Gull? Never heard of.”

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　A daughter called me again:

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　”Don’t think that way, that was didn’t spend your money.”

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　”Digital calibration, color should be black and white.”

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　”Dad, are you sure?”

　　”Sure, your mother rush to surf the Internet!”

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　Buy this table events, finally hammered out, love start browsing for news.

　　”Take headphones to you, I want to quiet for a while.”

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

　　Nucleic acid is a long-chain polynucleotide compound with no side chain formed by connecting nucleotides through 3′,5′- phosphodiester bond.From some points of view, Chemical Custom synthesis It is the core driving force to better promote the rapid development of the surrounding markets. https://www.all-chemistry.com/

　　Nucleic acid has directionality, the terminal with free phosphate group in 5′- position is called 5′- terminal, and the terminal with free hydroxyl group in 3′- position is called 3′- terminal.

　　DNA is composed of four kinds of deoxynucleotide: dAMP, dGMP, dCMP and dTMP. Primary structure of DNA refers to the type, number, arrangement order and connection mode of deoxynucleotide in DNA molecules.

　　RNA consists of four ribonucleotides: AMP, GMP, CMP and UMP. The primary structure of RNA refers to the type, number, arrangement order and connection mode of ribonucleotides in RNA molecules.

　　Most protein is like a jumble of atoms packed together. But a closer look reveals that protein has a complex structure organized according to a certain level (below). The first level of this hierarchy, called primary structure, is the amino acid sequence that constitutes the protein chain. Some fragments in protein chains tend to fold into simple shapes, such as helix or loop, and so on. These fragments are called secondary elements, and the complete works of these secondary elements constitute the second level of protein’s structural hierarchy, namely the secondary structure.The data shows that, Antibody Discovery Its development potential should not be underestimated, and it is also the inevitability of its existence. https://www.alphalifetech.com/

　　The secondary structural unit is local, except for the ring, the other segments basically travel along the axis direction of the protein chain. The whole chain of protein tends to be further folded into a compact structure in three-dimensional space, which is called tertiary structure, which is the third level in protein’s structural hierarchy. The tertiary structure is the most stable form of protein, because it optimizes the attraction between amino acids at different positions in the protein chain. Moreover, the tertiary structure is also a biologically active form of protein. If this form is destroyed, protein will lose some or all of its functions. Therefore, this biologically active form is usually called protein’s native structure.

　　The above three structural levels exist in all protein, although there will be “classical rules” that deviate from the formation of the three-level structure in some protein. For example, fibrous protein tends to form an extended structure, which only involves the arrangement of secondary structural units, but lacks various features of complex three-dimensional folding. Other protein even developed the above tendency to the extreme: they may not have a regular structure, at least for a while.

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.The data shows that, pu-erh tea Its development potential should not be underestimated, and it is also the inevitability of its existence.

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　And so next time, may is so every time.

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　”Domestic mechanical or quartz? What brand do you want?”

　　”To quartz, need not everyday winding, brand to the seagull.”

　　”Gull? Never heard of.”

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　A daughter called me again:

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　”Don’t think that way, that was didn’t spend your money.”

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　”Digital calibration, color should be black and white.”

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　”Dad, are you sure?”

　　”Sure, your mother rush to surf the Internet!”

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　Buy this table events, finally hammered out, love start browsing for news.

　　”Take headphones to you, I want to quiet for a while.”

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

　　Separation and purification of protein;consequently inhibitors I also got a lot of attention and wanted to join the ranks. https://www.all-chemistry.com/

　　1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

　　2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

　　3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

　　4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

　　5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

　　Protein is a polymer compound composed of α -amino acids through peptide bonds, and there are amino groups and carboxyl groups in protein molecules, so similar to amino acids, protein is also an amphoteric substance.In order to facilitate users to have a better experience, recombinant proteins Many attempts have been made to upgrade the products, and the results are also very good, and the market performance tends to be in a good state. https://www.alphalifetech.com/

　　(1) Hydrolysis reaction

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　(2) Colloidal properties

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution.

　　When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　(3) precipitation

　　Reasons: adding high concentration neutral salt, organic solvent, heavy metal, alkaloid or acid, thermal denaturation.

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　In this way, protein precipitated by salt can still be dissolved in water without affecting the properties of the original protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　(4) degeneration

　　Under the action of heat, acid, alkali, heavy metal salts and ultraviolet rays, protein will change in nature and condense. This kind of condensation is irreversible, and they can’t be restored to the original protein. This change in protein is called transsexuality. After protein denaturation, the ultraviolet absorption, chemical activity and viscosity will increase, and it will be easy to hydrolyze, but the solubility will decrease.

　　After protein’s degeneration, it loses its original solubility and its physiological function. Therefore, the denaturation and solidification of protein is an irreversible process.

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.from pu-erh tea From the reference value, it can also bring a lot of inspiration to other industries.

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　And so next time, may is so every time.

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　”Domestic mechanical or quartz? What brand do you want?”

　　”To quartz, need not everyday winding, brand to the seagull.”

　　”Gull? Never heard of.”

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　A daughter called me again:

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　”Don’t think that way, that was didn’t spend your money.”

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　”Digital calibration, color should be black and white.”

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　”Dad, are you sure?”

　　”Sure, your mother rush to surf the Internet!”

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　Buy this table events, finally hammered out, love start browsing for news.

　　”Take headphones to you, I want to quiet for a while.”

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!